LSPrimer accepts a multiple squences alignment file that containing target and nontarget sequences as input, and picking a set of PCR primers that will sensitively and specifically amplify the homologs accross the target squences.
Please past your alignment file in the box below, or upload it directly. Your alinment file must containing target and nontarget sequences. Target sequences are the homologous sequences of a lineage. Nontarget sequences are the closely related sequences that are not belong to the target lineage. Alinment file must be FASTA format. Moreover, you must specify which sequence to be the template for primer design in the red box.